All ethanol extraction procedures thereafter were conducted in a cold room (4°C).
The slides were incubated in a humidity chamber for 1 h at 37°C, washed (see washing procedure), and thereafter 40 μL ligation solution was added to each sample.
In a blinded, controlled study, we recorded the MIR in 160 consecutive patients (49 F, 111 M; mean age 44.4 ± 13.5 years) who underwent dental or surgical procedures and thereafter reported sensory disturbances in the territory of the mandibular (IAN or lingual) nerves (third molar extraction: 81; dental implants: 37; mandibular surgery: 22; multiple procedures: 20).
Surgical procedures have been classified according to a Danish classification system until the end of 1995 and according to a Danish version of the Nordic Classification of Surgical Procedures (NCSP) thereafter.
Standard acoustic and other tests are performed in a sound-treated room before the procedure and monitored thereafter.
Quality-of-life estimates are calculated monthly within the first year following procedure and annually thereafter.
Thereafter, procedure was followed by manufacturer's instructions using a commercial ELISPOT assay kit (MABTECH, Mariemont, OH, USA).
In the absence of these complications, patients were discharged from hospital on post procedure day 3. Thereafter, patients were followed up at the out-patient clinic on post procedure day 7 with liver biochemistry, to detect delayed appearance of complications.
Angiographic follow-up and neurological examination with emphasis on intermittent ischemic events was performed at 6 weeks, 12 weeks, 6 months, and 12 months following the procedure and yearly thereafter.
17 We assumed that if symptoms do not recur within two years of the initial ablation, then they are unlikely to do so later, and therefore no repeat procedure takes place thereafter.
