At this procedure the samples were heated under flowing helium in a graphite crucible up to about 2700 °C.
At the end of the deposition procedure, the samples were rinsed in 1× PBS and subsequently maintained in cell-culture medium at 37°C.
During the sonication procedure the samples were continuously cooled on ice.
After preparative procedure, the samples were lyophilized using the equipment TermoFisher FR-Drying Digital Unit (Scientific).
Due to the screening procedure the sample was reduced at each time.
By this procedure, the sample size of effects in lower hierarchical steps becomes fairly small.
Following this procedure, the samples were centrifuged at 8000 g for 10 minutes at 4°C.
As indicated in the experimental procedure, the samples were taken simultaneously on different plants that had undergone various stress treatments.
Following the ethanol washing procedure, the samples were then treated with a 50 : 50 solution of ethanol : amylacetate for 30 minutes.
Since only patients with stable menstrual cycles would be included in this procedure, the samples may be more monotonous.
