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At this procedure the samples were heated under flowing helium in a graphite crucible up to about 2700 °C.
During the degradation procedure, the samples were stirred in a 50-mL beaker containing 40 mL of MO aqueous solution (20 mg/L) with no oxygen bubbles.
Beginning with the dip-coat procedure, the samples were dipped at a speed of 60 mm/min without stopping time into a 0.6% release-agent solution.
After this procedure, the samples were treated with ethanol/water in 1 1 (v/v) proportion, dried in a stream of nitrogen gas, and baked for 15 min at 110°C in order to dry completely.
At the end of the deposition procedure, the samples were rinsed in 1× PBS and subsequently maintained in cell-culture medium at 37°C.
During the sonication procedure the samples were continuously cooled on ice.
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Due to the screening procedure the sample was reduced at each time.
By this procedure, the sample size of effects in lower hierarchical steps becomes fairly small.
In the split-sample procedure, the sample dataset is randomly split into two subsets: a training set for model building and a test set for performance assessment.
During measuring procedures, the samples were treated at 300 °C overnight under vacuum.
Following the nucleic acid purification procedures, the samples were stored at −20°C until use.
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