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For cloning PCR procedure, the protocol was similar to that described above except a tiny amount of bacterial colony was used to replace the template DNA.
In the case of BMD this is due to a failure in the μCT procedure: the protocol defined for analyzing samples of mild demineralization did not converge for the 40-hour sample.
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For May-Grünwald Giemsa staining procedure, the protocols used to obtain brain sections (40 µm thick) were described elsewhere [53], [28].
We have shown the device procedure and the protocol messages designed for its proper operation.
The cannulation procedure followed the protocol that was reported previously26.
The procedure followed the protocol described in the previous publication [50].
The enzymatic COMT assay procedure followed the protocol described by Masuda's group [74].
The purification procedure followed the protocol developed by Pinotti et al. [ 44].
The staining procedure followed the protocol described by Campion et al. (Campion et al., 2012).
Briefly, the first immunoperoxidase procedure used the protocol for single labeling described above.
The ethics committee of Chinese University of Hong Kong approved the recruitment procedure and the protocol described in this study.
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