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Without this validation procedure, the extracted blood is only given by the samples for metabolite analysis.
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For pooling procedures, the extracted data were combined across treatment arms rather than across individual trials.
To our knowledge, some laboratories performing PCR analysis for routine diagnostic procedures store the extracted DNA at -20°C [ 25].
In another study with different extraction procedure, the extracts of B. javanica fruits (ethanol, MeOH-ethanol, aqueous-MeOH residue, ethyl acetate and ethyl alcohol extracts) have been shown to have a promising antiplasmodial activity against P. falciparum K1 strain (IC50 < 10 μg/ml).
Assuming no metabolism and no recovery loss during the extraction procedure, the undiluted extract and the 1000-fold diluTextractsracts would theoretically result in well concentrations of 78 μmol L−1 and 78 nmol L−1 T, respectively.
A control was prepared using the same procedure replacing the extract with distilled water.
After repeating this procedure twice, the extract was divided into two aliquots and the solvent evaporated under nitrogen at room temperature.
To check complete recovery, this procedure was repeated once on the extracted sample.
In the course of the classification procedure, we extracted the sequences belonging to each gene family irrespective of the presence of tCWCH2 motifs identified by BLAST search using representative entire amino acid sequences.
ACC was extracted by a solid-phase extraction procedure using half the extract [ 80].
We then repeated the procedure, extracting the interactors of the interactors identified in the previous round, continuing until no new PRO IDs were found.
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