Sentence examples for procedure the column from inspiring English sources
The RNA was washed on the column (according to the RNEasy Mini kit procedure), the column dried (11000 g, 1 min, RT) and the RNA eluted in 60 μl of nuclease free water (Qiagen, Germany).
Open image in new window Fig. 3 Flowchart of the framework of the form-finding procedure (the right column shows the calculations in each iteration; the left column shows the updated state variables for calculation).
Only when using the combination of the tandem SPE sample clean-up procedure and the column-switch LC MS/MS system, the matrix effects are sufficiently decreased, enabling detection and identification of all antiviral drugs included at relevant levels.
For example, in classical Greek architecture, the proportions of the components of columns are precisely spelled out for each order, but this does not provide a step by step procedure for building the column.
Binding in a batch procedure and subsequent packing of the resin-protein complex into a column seemed to be much more efficient compared to the column procedure.
It clearly appears that the bands corresponding to the 4S and 5S RNA species are almost completely absent in the samples purified by the column procedure (C).
These histograms run pretty close one against each other proving that our random procedure can essentially reproduce the column evolution that exists in nature.
Advantages of the column procedure are: no target coating is needed for each round as the column is re-usable, no post-coating is needed, the contact time between the phage-containing solution and the column is shorter and, finally, a milder elution medium can be used.
Blood serum sampleswere taken before and immediately after the procedure, column lavages were taken as well.
The procedure gives structural information of the column, and therefore iterative computation encountered in the design using conventional procedure and commercial packages can be eliminated.
Only 1 round was necessary to obtain high-affinity binders in the column panning procedure, compared to 3 rounds in the tube panning procedure.
