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The principle of the extraction procedure (solvent, salt) was not changed, to avoid the possibility invalidating data acquired over past decades.
Procedure solvent blank samples representing every seventh sample were analyzed in the same way as the serum extracts Limits of quantification (LO Qs were defined in direct relation to the amount of interference of PBDEs in the blank samples.
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The promising solvents were proposed through a comprehensive procedure of solvent selection from solvent screening to complete process design.
The procedure for solvent-solvent separation was adopted from Ranjan [ 15] with minor modifications.
Generally, the test procedure for solvent tolerance characteristics of bacteria is determined by exposing a solvent to high-density late-exponentially grown cells, as described earlier.
The developed analytical procedure is solvent free, cost effective and fast.
A single run of traditional density modification procedure, including solvent flattening, histogram matching, and two-fold NCS averaging resulted in the phases with a figure-of-merit (FOM) weighted mean phase error of ~63° (Fig. 2B: Cycle 1).
The methodology proved to be a suitable, simple and flexible procedure for solvent selection at the initial stages of the crystallization operation design, being able to be upgraded for advanced stages of the crystallization process development.
These phases were then used in a density modification procedure involving solvent flattening and two-fold NCS averaging (without recombining the modified and starting phases) using DM.
The sample preparation procedure and solvent impurities can introduce ions unrelated to the analyzed matrix.
In this procedure, toxic solvents, reducing agents, and stabilizing agents are avoided.
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