A three lot validation study was performed [ 20] in which the following was validated: cell separation procedure, freezing media, dispensing effect on function, freezing procedure, and shipping on dry ice.
He observed "a general slackening off in procedure; ships are seldom where they should be, and a minority of merchant ships identify themselves to aircraft".
Details of the procedures for shipping samples, sieving, batching, and quality control of the laboratory analyses have been described (Colt et al. 2004).
Serum TSH levels were measured by immunochemiluminiscent procedures (SHIP-1: Immulite 2000, Third Generation, DPC, Los Angeles, USA; SHIP-Trend-0: Vista, Siemens, Eschborn, Germany).
DNA was prepared from peripheral blood by standard phenol chloroform procedures and shipped to Affymetrix (http://www.affymetrix.com) for genotyping (full data under preparation for publication).
Samples were processed following standardized procedures and shipped on dry ice to the Northwest Lipid Metabolism and Diabetes Research Laboratories, University of Washington Seattlee, WA).
Samples were processed immediately according to standardized procedures and shipped on dry ice for analysis at the TODAY central biochemical laboratory (8).
The additional handling procedures also slowed shipping times.
As a result, C57BL/6J islets isolated at Vanderbilt were first cultured for 24 h and then shipped, by the procedures used for shipping human islets, to the University of Massachusetts, which then returned them by overnight courier to Vanderbilt.
Management practices may vary among studs; differences in handling, cooling and shipping procedures together with variable semen quality of different stallions make fertility data difficult to compare between regions and individuals.
