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In a two-step procedure, seeds were removed and the remainder of the fruit was ground and mixed with apple juice, citric acid solution and sugar in a mixing tank with stirring.
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The cells were resuspended in fresh medium, diluted to 10 cells/ml using limiting dilution procedures, seeded into 96-well plates (0.1 ml/well, contained about one cell), and cultured in DMEM medium containing 10% FBS.
In this procedure, metal seeds the size of a grain of rice are permanently inserted into the prostate through needles.
It is a common practice in sugarcane micropropagated biofactories to perform a disinfection procedure to seeds that decreases the natural population of microorganisms and may be an opportunity to introduce selected microbiota.
See Methods section for specific procedure on seeding and analysis of coverage on seeded substrates.
The procedure for seeding and incubation of Vero cells was explained in details in previous research [11, 23, 27].
The procedure involved seeding of the capillary tube with zeolite nanocrystals followed by controlled zeolite regrowth and crystallization under flow conditions.
In typical procedure, the seeding process was carried out via in situ reduction of gold salt on the substrate sample [45].
In the second experiment, 40 slice images from CT thoracic studies of 10 subjects were used to segment the liver following the same procedure for seed selection (Figure 8).
The current strategy is implantation of a biodegradable scaffold and filling the porous architecture with either adipose tissue from a fat grafting procedure and/or seeding it with adipocyte precursor cells.
Since the original concentration of applied cells is known in an ACI procedure, and seeded in a confined volume, it is possible to quantify the total amount of labeled cells and follow them in the same patient in subsequent MRI sessions.
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