Sentence examples for procedure sections from inspiring English sources

For this procedure, sections were incubated in cryoprotectant solution containing 25% sucrose and 3.5% glycerol in 0.05 M PB at pH 7.4 and subsequently rapidly immersed in chlorodifluoromethane followed by liquid nitrogen and then thawed at room temperature in PB.

Upon completion of in situ hybridization procedure sections were treated for antigen retrieval using either incubation in 1% SDS in PBS at room temperature for 5 min or by boiling in a pressure cooker for 10 min in Tris-EDTA buffer (40 mM Tris, 0.1 mM EDTA, pH 8).

In each procedure, sections of thymus tissue served as positive control because apoptosis is a constant event in this organ.

For immunohistochemistry procedure sections were rehydrated, then delineated with a Dako pen, and treated with 0.05% trypsin (Sigma-Aldrich) for 20 minutes at 37°C.

At the end of this procedure, sections were counterstained with Mayer's hematoxylin, dehydrated, mounted on the slides and examined with a microscope equipped with a charge-coupled device camera.

See "Population and procedure" section.

Solution algorithm is presented in the 'Solution procedure' section.

"The RMPA handover procedure" section describes the RMPA handover design and related procedures.

For additional details, see "Inversion Procedure" section in Additional file 1.

'Solution procedure' section introduces the hybrid genetic search algorithm to solve the problem.

The samples were analyzed using the conductometric measurement system (see the "Measurement procedure" section).