Even though no genomic rearrangement had been detected by sequencing, the organisation of all the sequences surrounding dRS3 repeats, potential targets for the MDA phage, was confirmed using PCR and primers specific for each of these sequences (see the experimental procedure section for details).
Participants were asked to complete measures at two time points (see Procedure section for administered measures).
It is also used to call procedures or ranges of procedures (see the procedures section for more details).
To do so, in the first experiment, we compared the amount of looking that vervets accorded to each of two rhesus monkey facial expressions made when vocalizing two different calls a coo or a grunt in the presence of the audio version of one of these calls versus looking at the same faces in silence (Figure 1A; please see the Experimental Procedures section for more details).
In the phylogenetic analysis, trees were constructed based on the sequence of each individual gene with sufficient homologs in the database (see experimental procedures section for details).
In the wt strain of P. pastoris approximately 300 proteins passed the criteria (see experimental procedures section for details), whereas about 150 proteins passed these criteria in the recombinant protein producing strain.
Three experiments were carried out, all of which involved visual images and auditory soundscapes (translations of visual images to sound through an SSA) that were delivered in the same experiment in a semioverlapping manner (Fig. 1, see Experimental Procedures section for detailed description).
After completing each of the conditions (single-task, IVIS, and OSPAN; see the "Procedure" section below for details) in the experiment, participants responded to the NASA TLX survey consisting of six questions that used a 21-point Likert scale, ranging from "very low" to "very high".
Therefore to circumvent the limitations describe above, we have simply replaced the radioactive detection part of the available method by a well standardized qPCR method (see Results and Experimental procedures sections for methodology).
Upon completion of in situ hybridization procedure sections were treated for antigen retrieval using either incubation in 1% SDS in PBS at room temperature for 5 min or by boiling in a pressure cooker for 10 min in Tris-EDTA buffer (40 mM Tris, 0.1 mM EDTA, pH 8).
For this procedure, sections were incubated in cryoprotectant solution containing 25% sucrose and 3.5% glycerol in 0.05 M PB at pH 7.4 and subsequently rapidly immersed in chlorodifluoromethane followed by liquid nitrogen and then thawed at room temperature in PB.
