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OM-S-treated sputa were successful in both protocols; if the Xpert buffer is not added during the H.2 procedure, sample viscosity may require repeat testing.
A mycotoxin sampling plan is defined by the mycotoxin test procedure (sample size, sample preparation method, and analytical method) and the accept/reject limit.
Experimental conditions affecting the DLLME procedure (sample pH, sodium chloride percentage, ionic liquid amount and volume of disperser solvent) were optimized by means of an experimental design.
After successful template removal, the parameters affecting the SBSE procedure (sample volume, ionic strength, extraction time and pH) were optimized for the effective rebinding of the target analyte.
These criteria include, e.g., reporting of elicitation procedure, sample size, anchor states, and others.
Each household member was then eligible for selection for the interview and examination procedure (sample person), some of whom were non-Hispanic individuals.
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During the TUNEL procedure samples were washed in PBS.
During the procedure samples were taken for both cytologic and histopathologic examination.
With this procedure, sampling continues until a previously defined number of complete interviews is achieved.
For urea-based procedure, samples were analyzed in technical duplicate (repeated injection of the same sample) and the results combined.
Before continuing with the FISH procedure, samples were post-fixed using 1 % paraformaldehyde/PBS for 15 min.
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