10, 26 Thus, we applied PCA without subtracting the average of the observations, a procedure already reported in the literature, 12 and which in our case led to the maximization of the strain energy accounted for by each mode.
SOP often includes scene procedures, sampling procedures, laboratory procedures, reporting procedures, storage procedures, equipment procedures, and safety procedures.
They were made according to the Fmoc procedure, as already reported for peptide α4, for its "stabilized" analogue i.e., peptide K156, and for the peptide INH5, derived from the α5 helix [30], [43].
Autoradiography (Additional file 1) was performed following procedures already reported [40].
DPA-714 was labeled with fluorine-18 (half-life, 109.8 minutes) at its 2-fluoroethylmoiety using a tosyloxy-for-fluorine nucleophilic aliphatic substitution, according to slight modifications of procedures already reported [ 30, 31], and using a commercially available GE TRACERLab FX-FN synthesizer [ 32].
The SnO2 NPs were synthesized using an already reported procedure [38].
The total flavonoid content in plant extract and fractions was determined by following the already reported procedure [32].
Preparation of functionalized XAD-4 polymer was carried out according to the already reported procedure (Solangi et al. 2009).
This procedure has already been reported in detail elsewhere[ 33].
The analytical procedure has already been reported in a previous study [ 12].
