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During the CD, BE was managed according to an internal procedure published previously.
To determine the levels of CAR.HER2-CD137ζ CAR.HER2-CD137ζ, real-time PCR was performed usingene ABI PRISM 7900HT Sexpressiontection System (Applied Biosystems) according to a procedure PCRlished previously (Till et al., 2012).
Western blotting was performed using the same procedure published previously.
The Cu radiolabeling procedure was conducted according to a slightly modified procedure published previously.
Estimates of caloric and protein intake were made following a procedure published previously.
Spherical gold nanoparticles with a diameter of 80 nm were synthesized as a reference system for SERS studies following a procedure published previously.
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18 A dye-swap reference design was conducted using three time points (80, 100, and 110) during late pregnancy according to procedures published previously.
RT PCR of RASSF1A was used as a positive control and was carried out according to procedures published previously (Lusher et al, 2002).
Microspectrophotometry with DAPI followed procedures published previously (Kapraun and Nguyen 1994; Kapraun et al. 2007) using a protocol modified after Goff and Coleman (1990).
The details of this procedure were published previously [ 28].
Detailed information about this procedure was published previously.
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