From the total RNA, mRNA was enriched using a bead-based selective capture procedure, which was previously shown to have a limited impact on the mRNA composition [ 11].
In fact, while endotoxemia in patients undergoing major abdominal procedures has been previously shown [ 13, 14], our observation extend to other kind of surgical interventions less likely to be characterized by endotoxemia.
Though these measures were not individually included in the analysis of WGS from Complete Genomics, the quality score procedure used here was previously shown to improve concordance to orthogonal validation by Sanger sequencing (Soyombo et al., 2013).
However, these factors cannot account for the discrepancy between previous studies because we controlled for reinforcers earned and used the same training procedure and random ratio schedule previously shown to be effective in producing satiety-induced devaluation in C57BL/6J-background mice [30].
2. Using our general quasi-steady-state-approximation procedure [ 56], we have previously shown that any irreversible trimolecular reaction can be understood as limit case of a reversible bimolecular reaction and another irreversible bimolecular reaction by introducing one additional intermediate [ 57].
However, it was previously shown that this procedure still does not provide sufficient information to uniquely determine all model parameters.
It was previously shown that this procedure allows us to quantitatively estimate changes in protein-level regulatory activity of RNA-binding trans-acting factors (Foat et al).
The sub-retinal injection procedure caused a localized retinal detachment which has been previously shown to resolve within several days post surgery [55].
This is the first time such a procedure to manipulate the microbial communities, or microbiomes, of newborns has been tried in humans, although it has been previously shown to work in mice, Clemente said.
