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In order to compare how FC decay rates vary with varying algal biomass in wastewater of different strengths, the procedure outlined above was repeated using low strength wastewater (LSW) (78 ± 19 mg L−1 BOD5) and medium strength wastewater (162 ± 46 mg L−1 BOD5) at the same time and conditions.
The passaging procedure outlined above was repeated each time the cells reached 80 90 % confluency.
The entire classification procedure outlined above was performed 100 times with a different random permutation of the training labels for each classification type for each participant.
The ELISA procedure outlined above was conducted but after the transfected cells were fixed with 3.7% formaldehyde for 15 min, the cells were permeabilized with 0.1% Triton X-100 in PBS for 1 h.
The replication process followed the two-fold procedure outlined above.
The teachers' self-report was consistent with the procedure outlined above.
The valuation procedure outlined above can be referred to as the arbitrage-free pricing paradigm.
Following the procedure outlined above the volcanic ash attenuation relationship for the best-fit FALL3D simulation was considered and an ALPE was derived for this event.
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Approximately 36 hours following transduction, cells were lysed with RIPA buffer and western blotted according to the procedures outlined above.
The three main geographic locations of sampling (Metropolitan area of São Paulo, Ribeirão Preto and São José do Rio Preto) were coded as multistate characters and the most parsimonious description of geographic spread was obtained by MCMC sampling from the plausible set of trees using the procedures outlined above.
Field data collectors who were approached for unscheduled evaluations followed the same procedures outlined above.
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