Suggestions(2)
Exact(3)
Finally, three days later the procedure of virus concentration was repeated for the third amplification and 50% of cells were used to infect 40100 cm dishes.
The adenoviral stocks could be stored at -70°C over months without loosing their infectious capacity by -70 C. The procedure of virus generation is non-laborious and suitable for high-throughput applications.
5 7 days later the same procedure of virus extraction was performed, the pellet was resuspended in 14 ml of PBS and an additional centrifugation by 10.000 rpm for 30 minutes was introduced after freeze-thaw cycles.
Similar(57)
These viruses were isolated by serial passage in bovine turbinate cells (ATCC CRL-1390) using standard procedures of virus isolation in cell culture.
The main advantage is the capacity to discover essentially everything that is 'out there', even at low abundance, without the need for the laborious and biased procedures of virus and host growth.
Although the use of viral transport medium should ensure that virus will maintain viability under refrigeration, we pre-tested the effect of transportation procedures on virus viability by using a sample of viable human influenza virus in virus transport medium (provided by ProvLab) in 2 pre-tests (including courier to Memphis and culture in Memphis).
While Brussaard and colleagues [ 32] provided a detailed discussion of FCM procedures for virus detection, this area remains partly unexplored as previous studies were predominantly focused on marine ecosystems.
The procedure of gel filtration of virus samples recovered 90 and 180 minutes after drying did not reduce the infectious titers (data not shown).
Laboratories of the GPLN use standard procedures for virus isolation, identification, and intratypic differentiation (2 ).
The identification and isolation of virus strains was performed according standard procedures.
These assays provide sensitive and rapid alternatives to supplement the routine procedures of ELISA and virus isolation for SVDV diagnosis.
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