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Fig. 3 Procedure of tissue dissection.
For non-stressed control mice (n = 6 for each genotype), the same procedure of tissue collection as in stress-treated mice was used.
The procedure of tissue handling is shown in Fig. 1.
The procedure of tissue immunohistochemistry analysis for CtBP and SIRT4 was described elsewhere.
Moreover, the invasive procedure of tissue sampling limits the utility of this procedure for epidemiologic studies.
On the other hand, the procedure of tissue dissection will destroy the integrity of cells especially in an aqueous environment, meanwhile, endogenous RNases will be released and have much chances to connect with RNA.
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21 Detailed procedures of tissue handling and dissection are described further in Data S1.
The most ethically sound basis to oppose tissue harvest is that it imposes risks of bodily injuries associated with the medical procedures of tissue harvest.
The potential implications are on the procedures of tissues characterization and on the design and analysis of artificial tissue scaffolds.
Although desirable, standardization of routine tissue handling procedures and of tissue localization methods does not appear imminent due to clinical and practical considerations, which differ by laboratory, and the absence of compelling evidence that such standardization will be of value.
Although the freezing-thawing procedure of ovarian tissue is now relatively well established, the use of the cryopreserved ovarian cortex in order to restore fertility remains a challenge.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com