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Furthermore, in order to standardize the procedure of strain calculation, the ROI chosen was placed on the full thickness of the anterior cervical lip.
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The remaining stress was reduced by the procedure of additional strain loading and unloading.
The adaptation procedure of the strain S. cerevisiae DQ1 was described in detail by Zhang et al. [ 13].
The identification procedure of 12 strains was repeated once to assess the reliability of the API 20NE system.
Based on a technical engineering theory an analytical procedure for calculation of strain energy release rate and its separation into modes I and II of delamination is presented.
In the optimized inoculum preparation procedure, 10 CFU of strain M145 spores [typically corresponding to approx. 1 mL of a thawed spore stock in 20% (v/v) glycerol] were germinated for 5 hours at 30°C and 250 rpm in 250 mL baffled shake-flasks with 2 g of 3 mm glass beads and 50 mL 2x YT medium [ 26].
The FRAP assay was carried out according to the procedure of Benzie and Strain [ 36] with slight modification.
Ferric reducing antioxidant power (FRAP) assay of different fractions of F. palmata extract was done by following the procedure of Benzie and Strain [ 26] with some modification [ 27].
In this work, we used four different strains obtained after this procedure: Two pairs of strains with and without Bari-Jheh.
The fused linear fragments were purified and used in separate transformation procedure of chemically competent yeast strain CEN.PK 113-5D.
The software is based on a procedure of minimization of nonlinear strain energy for a given constitutive model of the material (Bonet and Wood, 1997).
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