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Such accumulation was not observed in the wild-type and Appl null mutant brains by the identical procedure of staining and observation (Figure S3).
Due to the preferred procedure of staining FNAC to determine ER, patients with ER results available had more often FNAC done for their pathological diagnosis (Table 2).> -wrap-foot> +fine – fineedledle aspiration cytology.
The procedure of staining collagen type II was similar to the protocol mentioned above with the following changes: monoclonal mouse antirat collagen type II antibody (1 200 dilution, Fisher Scientific, Chicago, IL, USA) was applied as the primary antibody, and anti-mouse IgG/HRP (Fisher Scientific) as the secondary antibody.
The procedure of staining was performed as described elsewhere [15].
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The procedures of staining, dehydration, clearing, infiltration, and embedding were performed according to Liu et al. ([2005]).
Furthermore, to decrease the staining bias, we used automated machine for all procedure of IHC staining.
With further development of antibodies and refinement of staining procedure, we carried out immunohistochemical analysis for the expression of GATA4 and GATA6 in a panel of ovarian non-neoplastic and cancer tissues in tissue microarray (Table 1).
In fact, the fundamental basis of pathologic diagnosis has remained essentially unchanged for more than 100 years, following the standardization of staining procedures such as hematoxylin and eosin (H & E) for tissue sections and Wright-Giemsa staining for blood samples.
However, there are no published studies about optimization of staining procedures for a specific plant family or taxonomic group.
To confirm the specificity of staining all procedures were also performed without the first antibody.
We optimized eight-color antibody panels and procedures for staining and lysis of whole blood samples, and implemented pre-analytic steps with a semi-automated workflow using a robotic system.
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