The procedure of silver-enhanced LFIA for the detection of PCT was similar to that of the conventional LFIA.
The procedure of silver-enhanced LFIA based on 20 nm GNSs was performed with silver enhancer kit according to the manufacturer's instruction.
The 2 mL silver seeds (Synthesis according to the procedure in "Generation of Silver Nanoparticles") was added into the gelatin solution.
Similarly, silver doped glass was produced by repeating the same procedure followed by addition of silver nitrate solution before the addition of 1 M ammonia solution.
A procedure involving 0.1 equiv of silver(I) acetate, carried out using the benzaldehyde imine of l-alanine isopropyl ester, was found to proceed in 40 88% yield and with high product purity after chromatography, however, subsequent hydrolysis of the isopropyl ester was found in our hands to be problematic.
Our procedure using silver staining of 2-D gels allows the detection of proteins recruited in relatively low amounts (a few ng) in the affinity chromatography step.
Amplification products were stained using the DNA silver staining procedure of Promega, USA, following the manufacturer's instructions.
In addition, a selected number of frozen gelatin sections was collected in 4% paraformaldehyde, and processed with a modified Gallyas silver impregnation procedure of Nadler and Evenson [ 43] that selectively labels degenerating neurons and their processes.
The silver staining procedure of the yolk epithelium revealed brownish patch indicating large amounts of proteins on a restricted surface (Fig. 3B,C) which could correspond to ionocyte-like cells.
Nevertheless, the synthesis of a monodisperse and stable silver nanoparticle suspension is challenging and may go through tedious and complex procedures, which may hinder the practical applications of silver nanoparticles on textiles.
Fig. 1 Schematic procedures for fabrication of BNNN TCE. a Deposition of silver ink film on the tSi surface.
