This procedure of selective oxidation of diamond film is well established in the diamond research field and in the literature [5].
The procedure of nitrobenzene oxidation of lignin was conducted as follows; 0.05 g CWR was added with 5 mL 2 M NaOH and 0.5 mL nitrobenzene, and a stir bar was put into a 25 mL Teflon gasket in a stainless steel bomb.
The procedure of nitrobenzene oxidation of lignin was carried out as follows: First, 0.05 g CWR was added with 5 mL 2 M NaOH and 0.5 mL nitrobenzene, and a stir bar was put into a 25 mL Teflon gasket in a stainless steel bomb, and the bomb was sealed tightly and heated at 170°C (oil bath) for 3.5 h and stirred at 20 rpm.
Healthful foods rich in antioxidants may help slow down the process of oxidation.
Iron acetylacetonate complexes anchored on oxidized carbon nanofibres (CNFs) were prepared by a three steps procedure: (i) oxidation of commercial CNFs by treatment with HNO3, (ii) synthesis of acetylacetonate (acac) functional groups on the oxidized CNFs surfaces (acac/CNFs) and (iii) iron ions complexation on the acac sites of the CNFs (Fe-acac/CNFs).
During this procedure, the oxidation levels of modified HDL were measured (Additional file 1: Figure S1).
After the chemical procedure of the RC binding (surface oxidation, silanization, attachment of the RC protein through the GTA linker), the AFM image showed a more uniform slick surface (Figure 1B), with a higher surface roughness, RMS = 15.7 ± 0.5 nm.
The remainder of the procedure, oxidation with cholesterol oxidase and GP derivatisation followed by SPE purification, was performed as previously described with the exception that Sep-Pak C18 cartridges were replaced by Oasis HLB cartridges [11 13].
In this work, CoSe2 nanoparticles embedded in defective carbon nanotubes (CoSe2@DC) was synthesized by a carbonization-oxidation-selenylation procedure of Co-based metal-organic frameworks (MOFs).
19, 27 Thus, this general procedure of in situ HRP-mediated quinone oxidation, followed by conjugate addition, has substantial scope for further development into assays for a wide range of substrates.
