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An aliquot of cDNA was subjected to 35 cycles of PCR using a standard procedure of initiation at 65 °C for 5 mins, incubating at 37 °C for 2 mins, and inactivating at 70 °C for 15 mins.
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The post-process based on microscopic characterization using SEM technology allows to observe the procedure of damage initiation and its evolution.
The procedure of crack initiation and propagation has been comprehensively studied for blasting applications, and the role of different effects has been determined through numerous experimental and numerical investigations which will be briefly discussed in this section.
Firstly, we do not need the initial conditions and the procedure of re-initiation as we directly calculate the minimizer on the discrete gray level sets rather than solving the Euler-Lagrange equation of the underlying variational problem.
Moreover, much more details in the procedure of damage initiations and propagations, including fiber broken, matrix fracture and delamination, could be revealed.
Each mystic order claimed a chain of spiritual descent (silsilah) from the Prophet Muhammad, established procedures for initiation of members (murid, ikhwan, darwīsh, fakir), and prescribed disciplines.
These points were improved by Xu et al. (2015a, b) who presented a numerical procedure for prediction of initiation and evolution of fatigue damage on the level of a unit cell of textile composites under tension-tension loading.
He says he has always been in favour of "the power of initiation", a procedure that allowed a backbencher to stand up and move a motion for debate.
The primary objective of this study was to asses whether a recruitment procedure of the lung, at initiation of HFOV by stepwise increases of continuous distending pressures, impairs the hemodynamics and organ blood flow in lung-injured animals.
Transition to HFOV requires a recruitment procedure of the lung at initiation, typically performed by slow stepwise increases of continuous distending pressure to optimize the alveolar volume available for gas exchange, as used in several clinical studies [ 5- 8].
aThese guidelines assume that the procedures listed (with the exception of initiation of cardiopulmonary resuscitation [CPR]) are non-emergency.
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