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This phenomenon also suggests to us an improved procedure of culturing A. cinnamomea by treating cultures with light radiation for ten days before placing them in the dark.
This assumption warrants questioning, as does the procedure of culturing male cells in media such as fetal calf serum, which contains large amounts of female sex-steroid hormones unless stripped in charcoal.
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In the 2011 German Shiga toxin-producing E. coli (STEC) O104:H4 outbreak, the pathogen was identified by the standard epidemiology procedure of isolation, culturing, and genotyping (Rubino et al., 2011).
The first-level grouping into cultured vs. non-cultured samples most likely reflects the profound impact of culturing procedures on the transcriptional profiles of the different cell types.
The PCR procedures can overcome the obstacles of culturing fastidious organisms, and may offer a more sensitive and time efficient method for evaluating middle ear effusions.
The procedures for culturing and identification of the isolates were explained in a previous work [ 36].
The article provides an overview of 2D cell cultures and 3D tissue models – their properties, basic procedures, conditions of culturing and applications.
The procedure of cell culture and treatment of nanoparticles was similar with the above phase of MTT assay.
The detailed procedure of zygote culture in vitro was performed according to Qin and Zhao [ 15].
We used the standard procedure of cross-culture adaptation to develop the Chinese version of the PedsQL™ 3.0 Healthcare Satisfaction Generic Module.
In the standard procedure of cross-culture adaptation, we strictly followed the PedsQL™ Measurement Model Translation to finalize the Chinese version.
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