Although the synthetic procedure and the recrystallization process of complex 1 were performed in methanol, a marked preference for coordination of water over methanol was observed and proved by single crystal X-ray determination.
Many previous studies demonstrated that the analytical procedure of highly complex environmental samples such as wastewater can be negatively affected by matrix impurities leading to significantly reduced recoveries for many classes of analytes [39, 40].
The same filtering procedure of 1j8h complex was applied, which corresponded to: 32D A 32D:B 52D:A 52D:B (100–101)D:P where A, B stand for α and β chain of HLA-DR4, respectively, D stands for β chain of TCR, P stands for huCollp261.
Perhaps, the multistep procedure of the complex formation involving a disulphide interchange between the two proteins might be altered in our experimental conditions.
However, for one thing, the scanning and image reconstruction procedures are of complex operations, in high consumption and with strong radiation.
Supporting Information (see footnote on the first page of this article): Materials and instrumentation, detailed synthetic procedures, characterization of complexes, NMR and HRMS spectra, sensor preparation, measurement setup.
Both the complexes were synthesized under similar synthetic procedures and structure of complex 2 was additionally determined by single crystal X-ray diffraction studies.
The efficacy paradox can come into play whenever complex interventions are tested against a control procedure and the control procedure implies some form of complex intervention.
