The procedure of cell culture and treatment of nanoparticles was similar with the above phase of MTT assay.
In their work, a cell zooming server which is a virtual entity in the network controls the procedure of cell zooming.
Procedure of cell treatments with chemicals at indicated concentrations was illustrated in Fig. 1A, including Vitamin C (VC, Sigma, A4403), -epigallocatechin gallate (EGCG, Sigma, E4143), Vitamin E VEE, Sigma, T1782), N-Actyl-L-cysteine (NAC, Sigma, A7250), Metformin (Met, Tocris, 2864), Rapamycin (Rap, Tocris, 1292), and Resveratrol (Res, Sigma, R5010).
The pretreatment procedure of cell cycle analysis was following the manual of Cycle TestplusDNA Reagent Kit (Becton Dickinson).
The detailed procedure of cell proliferation (MTT) assay and IC50 determination was described previously.
The procedure of cell purification is depicted in Additional file 2, Figure S2 and was adapted from previous publications [ 84- 86].
The quenching procedure of cell samples was based on the method developed by de Koning et al.[ 25].
The co-regulation network suggested that the loss of PXC1 function might retard the initiation of secondary cell wall deposition by prolonging the course of cell wall remodeling and reorganization during the procedure of cell expansion.
S100 β, also called calcium binding protein β, is a member of the S100 family, which is anchored at the cytoplasm and nucleus and participates in the procedure of cell cycle and differentiation.
Characterization of intracellular ice formed during the cooling procedures of cells significantly benefits the development and optimization design of cryopreservation or cryosurgery techniques.
