At the end of eight weeks, various hemodynamic parameters were recorded following the procedure of carotid artery cannulation.
Pancreatic islets were isolated by the collagenase method using the procedure of pancreatic duct cannulation and density gradient purification as described elsewhere [ 10, 11].
The procedure of mesenteric lymph vessel cannulation was performed as previously reported with modification [ 25].
During the sham procedure, cannulation of the biliopancreatic duct without infusion of glycodeoxycholic acid was followed by intravenous saline (1 ml/h, 6 h).
However, these similar treatments have already been established as safety procedures, and cannulation of the dissected SMA has been performed during other endovascular treatments.
The procedures of anesthesia, tracheal cannulation, sampling of expired gas, and BAL require considerable time, which interferes with the measurement of cough reflex sensitivity that should be performed at scheduled time points.
In the studies described herein, mice were subjected to highly precise and reproducible experimental T/HS (bleeding down to 25 mmHg without resuscitation) for 1 4 h using a computerized hemorrhage system described previously [21], to the sham cannulation procedure (surgical cannulation trauma, ST) for the same periods of time, or in completely non-manipulated animals.
Animals were either untreated (n = 6) or were cannulated and divided into four groups (n = 6 mice per group), subjected to 1, 2, 3 or 4 h sham procedure (surgical cannulation trauma only; ST) or 1, 2, 3 and 4 h of HS in addition to this surgical cannulation trauma (ST + HS).
While paramedics performed a range of procedures including intravenous cannulation, administration of analgesia and oxygen, most women required minimal intervention.
Results of cannulation and the incidence of complications were compared.
