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As with any other regression procedure, marker genotypes can be centered by subtracting the mean of the marker genotype or centered and standardized to a unit variance; that is, x ˜ i j = (x i j − 2 θ j ) / 2 θ j (1 − θ j ), where { θ j } j = 1 p are estimates of the frequency of the allele coded as one.
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In overall breeding procedure, marker-assisted selection was performed from generation F1 to MF4 (multiple-cross filial, MF) to screen the plants positive for Xa7 and Pi2.
Following this procedure, marker-wise approximate exact P-values were then calculated by dividing the number of observed permutations achieving an equal or higher test statistic for this marker than the actually observed one by the number of permutations performed.
For all procedures, marker clips or other marking modality should be considered for application to cavity side walls to aid radiation planning.
Next, we optimised the assay's sensitivity by introducing a dual-antigen immunomagnetic tumour cell enrichment procedure before marker gene quantitation and by refining the panel of marker genes as follows: cytokeratin 19 (CK19), human secretory protein p1.B (p1B), human epithelial glycoprotein (EpCAM; here: EGP) and mammaglobin (MmGl) (Molloy et al, 2008).
An alternative procedure for marker selection that takes MAF and distances of adjacent markers into account was proposed by Wang et al. [ 27].
A high level of reliability is demonstrated within the test and this is realised through testing procedure and marker variability.
A typical procedure for marker development employs genomic DNA.
Genomic library microsatellite enrichment is an efficient procedure for marker development in melon.
The GBLUP were estimated as in Ødegård et al. [ 6] using the BLUP estimation procedure of marker effects [ 5].
Here, we describe a procedure for marker enrichment and candidate gene identification that uses NGS in combination with a Bulked Segregant Transcriptome Analysis (BSTA) approach.
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