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This procedure is prone to errors and high variability due to individuals' fatigue, lack of training, and subjectivity.
This procedure is prone to wavelength limitation due to geometrical constraints.
Until recently the Rz (random zero) sphygmomanometer has been the standard for measuring BP in population studies, but the procedure is prone to errors (Staessen et al. 2000).
Our "validation" tests are never performed on RNA – only on cDNA, and for obvious reasons: it is not valid to optimize real-time PCR using RNA since the RT step in a One-Step procedure is prone to variable degrees of efficiency (or, one should assume it to be; recent seminars on real-time qPCR have stressed this), and the reverse primer is used up to an unknown extent during 1st-strand synthesis.
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This suggest that while early steps in the procedure are prone to Type I errors, the later steps reduce the number of Type I errors to a level below α = 0.05.
However, RCTs evaluating diagnostic procedures are prone to specific risks of bias and threats to the statistical power which may challenge their validity and feasibility.
Moreover, most individual studies have relatively small sample sizes, and hence prediction models trained on individual studies by using cross-validation procedures are prone to over-fitting, leading to prediction accuracies that are over-estimated and lack generalizability [ 10].
In addition, these results highlight the shortcomings of current vector detection methods: the need to acquire multiple biopsies using an invasive procedure that is prone to sampling error and is concomitantly impractical for repeated monitoring of the entire tumor.
It is an invasive and costly procedure, and is prone to complications, some minor, such as pain, others more severe with a recorded risk of death of 0.01% [ 9- 11].
These experiments, however, used conventional agarose gel electrophoresis for the physical-size-based separation of fetal and maternal cf-DNA species – a cumbersome, labour-intensive, inefficient procedure that is prone to contamination.
A potential explanation for the higher validation rate of the MonoPrep samples is that this procedure is less prone to the introduction of contaminant proteins such as keratins (Table 1).
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