We originally performed this procedure including all Pfam domains.
Collectively, the automated procedure including all the critical steps (Fig. 1A) takes 2 hours.
All data were non-parametrically normalized using a ranking procedure including all sample-probe values and each gene-sample pair was given a signed P-value based on the rank.
In the course of the multivariate analysis of the factors associated with shortened survival time, we used Cox's proportional hazard models, applying the stepwise (forward) model building procedure, including all covariates significant at 20% level in bivariate analysis.
Both streams will follow the same procedure including all quantitative measures completed via telephone, but women in the qualitative stream will be invited for additional interviews at the time points specified.
In multivariate analysis of the factors associated with survival after resection, we used Cox proportional hazard models applying the stepwise model-building procedure including all covariates significant at the 10% level in univariate analysis.
In linear regression models using a forward stepwise procedure including all potential confounders, only gestational age was related to Ca pump activities in study mothers (p = 0.14 and p = 0.10 for CaMc and CaM, respectively) and was also one of the considered predictive factors (p = 0.15) for newborn hair Pb.
A multivariate logistic regression analysis with backward elimination procedure including all predictors showing a p value ≤ 0.25 in the univariate analysis was undertaken to obtain an adjusted odds ratio (OR) with 95%% confidence interval (CI) and define which variables were independently associated with NIV failure.
From this step until the flow cytometric sorting, the entire procedure, including all staining and washing steps, was performed in phosphate-buffered saline (PBS)/BSA at either pH 7.4 or 6.0 [2.7 mM KCl, 137 mM NaCl, 10 mM sodium phosphate plus 20 g/L bovine serum albumin (Sigma); either set to pH 7.4 (selection rounds 1, 2, and 5) or to pH 6.0 (selection rounds 3, 4, and 6)].
The general solution procedure including all the problems instead of separately solving each problem is quite advantageous.
