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Tracking of transplanted cells has become an important procedure in cell therapy.
Dual color imaging of several GFP variant proteins is a very attractive prospect and has become a standard procedure in cell biology [ 7, 8].
To evaluate the critical effects of this passaging procedure in cell culture on gene expression, RASF were therefore cultured over several passages followed by cDNA array analysis for up to eight passages and comparison of early passages to higher culture passages was performed.
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Development of procedures in cell biology to regenerate plants from single cells or organized tissue, and the discovery of novel techniques to transfer genes to plant cells provided the prerequisite for the practical use of genetic engineering in crop modification and improvement.
CCK-8 reagent was used as the procedure described in cell adhesion tests.
There are three major considerations to take into account when performing biochemical procedures in cells cultured in 3D collagen gels.
The data transmission procedure in every cell is identical.
We did the procedure in my cell.
Tumor tissues from mouse xenograft studies or breast cancer patients were homogenized using gentle MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's procedure in a cell lysis buffer in the presence of proteinase inhibitor cocktails (Calbiochem, San Diego, CA, USA).
Biopsy, medical diagnostic procedure in which cells or tissues are removed from a patient and examined visually, usually with a microscope.
For many at the meeting the debate came down to where they stood on the value of preimplantation genetic diagnosis (PGD), a relatively new procedure in which cells removed from IVF embryos are screened for inherited mutations and only apparently healthy embryos are implanted.
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