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The procedure for suspension bead arrays was performed as described previously.
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Article 45 establishes the procedure for the suspension of a member state which violates its principles and values.
Another gap in the Commission's proposal is in the procedure for ending suspension, which is not dealt with at all.
The MTS/PMS (Promega Corporation, Madison, WI, USA) colorimetric cell proliferation assay was used for the Colo320 and Colo320R suspension-type and MTT test (Sigma-Aldrich, St . Louis MO, USA) for HT-29 and HT-29R adherent cells, in order to simplify the solubilization procedures for the suspension-type cell lines [ 65].
The procedure for sub-culturing the suspension was empirically optimised with a view to increasing the number of viable single cells in the suspension population.
A standard test procedure for seats with horizontal suspensions could be developed from a test protocol used in a recent inter-laboratory study.
[Of note, the procedure for preparing single cell suspensions from the xenografts had no statistically relevant influence on antigen expression (see Additional file 1: Figure S7).] It is not yet known to what extent single antigens are responsible for stem cell maintenance, thus we do not know the functional relevance of these cell surface markers.
In our case, the optimal procedure for establishing the single cell suspension was to start with approximately 2 g fresh weight of callus.
Those up for suspension are charged with "violating inspection procedures," while the others were called out for "inattention to duty," according to the paper.
The procedures for adaptation to suspension cultures were similar to those we have previously used for High Five cells.
Strips for total leukocyte counts were prepared using the cell suspension according to the procedure for milk.
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