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We have presented here a simple, versatile, and user-friendly procedure for sorting of monodisperse double emulsion droplets in which the activity of an intracellularly expressed enzyme is assayed in cell lysate.
As a new approach for selectively targeting and sorting cancer cells, this exceptionally simple method not only illustrates a straightforward, selective, and inexpensive procedure for sorting cancer cells, but will also lead to the application of nanoparticles, based on the spatiotemporal distribution of a specific enzyme, for disease diagnosis and treatment.
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Because the block is actually a discrete implementation in a digital processor, it requires numerical procedures for sorting and mathematical calculation. .
In the proposed multi-objective colliding bodies optimization (MOCBO) algorithm, the collision theory strategy as the search process is utilized and the Maximin fitness procedure is incorporated to the CBO for sorting the agents.
Furthermore, we design some procedures for conveniently sorting out the network units, so that the most important ones are evaluated first.
This information could be useful to optimise the standing tree measurement procedure or when acoustic tools are installed on harvesters for sorting logs based on stiffness.
Doctors are paid for seeing patients, not for sorting through more than 14,000 diagnostic codes and 4,500 procedure codes while a patient sits on the exam table.
A proper light is necessary for sorting.
Our systematic procedure for IgR sorting is largely dependent on the utilization of open source tools (e.g., TranstermHP, Fuzznuc, Rsat, NNPP, BlastN, Rfam, QRNA) Thus, this methodology could be readily applied to any annotated DNA sequence for which appropriate BLAST partners and promoter consensus sequences are available.
To minimize effects of sorting, we repeated the same procedure after sorting by the glomeruli data and used the means of slope and intercept for the final adjustment function (y = 0.9392425x + 1.17905).
The procedure for single cell sorting and subsequent PCR amplifications has been described previously [ 24].
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