The overall procedure for probe staining took about 2 hours, and the total volume of waste is less than 10 ml; while the conventional Masson's Trichrome and Picrosirius Red stain on OCT sections took about the same time, but required few more steps of staining and washing.
For probing the modeled psychophysical functions by SRPs, stimulus amplitudes, x, were selected according to an adaptive probing procedure 1 described in (Doll et al., 2014).
For details of our experimental procedures, for probe localisation and for image capture protocol, see [ 19].
This method, already used in previous works, allows a simple procedure for atom probe sample preparation [20].
Visual recognition (see below for procedure) was probed with 2 tasks—naming and cross-modal (word picture) matching, using the 64 black and white line drawings from the CSM battery (Bozeat et al. 2000; Adlam et al. 2010).
To verify the efficiency and specificity of our biotinylation procedure, we also probed for GluR1, a well-known cell-surface subunit of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) receptors.
To address this challenge, we have developed a procedure for high-throughput probing of mammalian sperm with 91 lectins on lectin microarrays.
To obtain a comprehensive profile of the accessible glycans on mammalian sperm surface, we have developed a procedure for high-throughput probing of mammalian sperm on a lectin microarray with 91 lectins.
The approach described above allows a rigorous separation of N probes of processes I and III and we have used a similar procedure for methyl-C probes.
Hybridization was performed at 42°C according to the standard Roche Applied Science procedure for digoxigenin-labeled probes.
Moreover, our procedure for age-associated probe identification is not highly affected by genetic background and platform differences.
