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The overall procedure for probe staining took about 2 hours, and the total volume of waste is less than 10 ml; while the conventional Masson's Trichrome and Picrosirius Red stain on OCT sections took about the same time, but required few more steps of staining and washing.
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For details of our experimental procedures, for probe localisation and for image capture protocol, see [ 19].
This method, already used in previous works, allows a simple procedure for atom probe sample preparation [20].
The same procedure was used for probe preparation for the VG-V subfamily of VicTR-B repeats (using clone c609 [GenBank: DQ139394]) and for VicTR-A clones c653 [GenBank: EU568805] and c666 [GenBank: EU568818].
Moreover, our procedure for age-associated probe identification is not highly affected by genetic background and platform differences.
The approach described above allows a rigorous separation of N probes of processes I and III and we have used a similar procedure for methyl-C probes.
Manual connectorization procedure for multifunctional fiber probes.
Hybridization was performed at 42°C according to the standard Roche Applied Science procedure for digoxigenin-labeled probes.
To address this challenge, we have developed a procedure for high-throughput probing of mammalian sperm with 91 lectins on lectin microarrays.
Here we present, as an example, our procedure for identifying mouse probes that are most likely to hybridize with human RNAs, the converse would follow similar steps.
To obtain a comprehensive profile of the accessible glycans on mammalian sperm surface, we have developed a procedure for high-throughput probing of mammalian sperm on a lectin microarray with 91 lectins.
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