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The procedure for normalization of input parameters is the same as of bubble point ANN algorithm.
The procedure for normalization of input parameters is the same as of bubble point pressure and B ob ANN algorithms.
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Procedures for normalization to internal standards and for expression of the resultant data were according to the amplification plot method of Peirson and colleagues [100] as implemented in DART-PCR Excel workbook (http://www.gene-quantification.info/bestkeeper.html).html
The procedure for data normalization should also be explained more thoroughly in the results section as this may significantly alter the apparent variance by PCA.
The procedure of normalization is illustrated in Figure 1A (for details, see Materials and Methods).
Normalization of XB testis cDNA (which is a procedure different from and unrelated to normalization of microarray data) was performed prior to 454 pyrosequencing in order to increase representation of genes with low expression; procedures for cDNA normalization and 454 pyrosequencing are described elsewhere [27].
Procedures for data normalization and assessment of statistically and biologically significant genes were performed as described previously [ 22].
Procedures for data normalization and assessment of statistically and biologically significant genes were performed as described by Benedito et al. [2008][ 52].
Differential expression was quantified using DESeq 1.10.1 [ 20] applying the built-in procedures for library normalization and estimation of variance and with Cuffdiff 2.0 [ 21].
By applying our procedure for stratification and normalization to tumor specimens on different aCGH platforms we compare performance with standard methods.
The β-actin gene was used as reference gene for normalization procedure.
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