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The procedure for mutation was conducted according to the manufacturer's instructions (MutanBEST, TaKaRa Biotechnology) and the mutants were sequenced for verification.
Although the allele specific qPCR is an efficient closed-tube procedure for mutation identification, this fluorescent based method still costs false positive results [ 9].
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For example, laboratory procedures for mutation scanning tests often involve a primary screen to determine which fragments carry mutations, followed by a second confirmatory test by sequencing to characterize the mutations present.
In accordance with previous studies (Krypuy et al, 2006; Do et al, 2008; Ma et al, 2009), we confirm that HRMA is a reliable, sensitive and rapid procedure for KRAS mutation detection.
The aim of this study was the exploration of the screening procedure for CF mutations employed in our laboratory as a first-line diagnostic instrument for Cystic Fibrosis.
The procedures for iterative mutation of S. mobaraensis genome with ARTP mutation breeding system purchased from Si Qing Yuan Biotechnology Co., Ltd.
When a number of different estimation procedures for passenger mutation rates were used, it was found that approximately 120 genes were more frequently mutated than the passenger rate, suggesting that these genes are more likely to carry driver mutations (so-called CAN genes).
To develop an accurate mutation analysis procedure for retinoblastoma gene (RB1) mutation, which is sensitive at the single-cell level, and to use in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) to achieve pregnancies without retinoblastoma.
The procedure of mutation is summarized as follows: Firstly, a chromosome is randomly extracted.
The procedure for hill-climbing mutation is given below.
Computationally, even before the added motivation as a consequence of the latter disease related finding, this gave rise to a procedure for detecting deleterious mutations using RNA folding predictions numerous times [ 32].
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