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The procedure for labeling with 43Sc is as easy as that in the case of 68Ga and 44Sc which makes it possible to use commercially available kits.
We therefore developed a procedure for labeling under aerated conditions.
Solely based on this study, electrotransformation is the preferred universal labeling procedure for labeling of Listeria strains with gfp when compared to conjugation.
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Procedures for labeling mAbs with Zr or I by using good manufacturing procedures have been established, and therefore these radiopharmaceuticals are being investigated for clinical use.
Although the general procedures for labelling of WBC with either In-oxine or mTc-HMPAO are similar, there are some important differences between the procedures.
The same procedure was used for labeling a single domain antibody (VHH) fused to the HA tag that was used as the positive control.
By using this novel and simple radiohalogenation procedure, higher overall radiochemical yields of astatination were obtained in comparison with the use of an arylstannane precursor and procedures of the litterature for labeling the same antibody.
Alternatively, the entire procedure for erythrocytes labelling, washing and imaging with BODIPY-lipids was conducted at 37°C.
When the entire procedure for erythrocytes labelling, washing and imaging of BODIPY-GlcCer was conducted at 37°C, rounded patches of micrometric size were undistinguishable, indicating that these domains are not a metastable state due to exposure at low temperature (Fig. S2).
We also introduced 15 nt of RNA sequence at the 5′ and 3′ of the aptamer-HLE (GCATACCGGATACGC and CCATAGGCATAGCGC, respectively) as part of the splint ligation procedure for end-labelling the RNA with Cy3 dyes (see below).
We used solid-phase extraction, cartridge-purified [F]SFB according to our long-term standard procedure [ 38], which we have optimized for labeling peptides [ 39].
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