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Fig. 2 Outline of the workflow for procedure for isolation and analysis of EVs.
The procedure for isolation and preparation of lung tissues is found to be crucial in order to preserve the anatomy and structure of the pulmonary compartments.
In the conventional bacterial culture test, the procedure for isolation and identification of E. tarda from diseased fish samples usually requires 2 to 3 days, including bacterial enrichment, plating onto DHL agars, sequential subculture, and biochemical characteristic test, which is time-consuming and tedious.
The procedure for isolation of L.s.-P from L. saccharina has been described earlier [11].
Following a general procedure for isolation of small vesicles, the medium of DMSO- or NeoA-treated cells was subjected to sequential centrifugations, ending in a 120,000 g ultracentrifugation step; samples of the supernatants and pellets from the various steps in this procedure were then immunoblotted.
The A. fumigatus ΔakuA strain AfS35, the procedure for isolation of conidia, and the composition of the yeast glucose medium (YG) and the Aspergillus Minimal Medium (AMM) have been described previously [10].abouraud medium, casein and pepton were purchased from Becton-Dickinson.
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We applied routine procedures for isolation of putative Salmonella isolates, with enrichment in Rappaport-Vassiliadis broth and injection into Xylose-Lysine-Desoxycholate (XLD) agar.
The detailed procedures for isolation of arterial rings and organ culture have been described previously (Huh et al. 2011).
In this chapter, we describe the key procedures for isolation of the oligosaccharides and the preparation of neoglycolipid probes together with expression of malectin that have enabled the discovery of the highly selective binding of this newly described protein in the endoplasmic reticulum (ER) to a diglucosyl high-mannose N-glycan.
Electroporation of S. suis and procedures for isolation of mutants were those described previously [50].
The feasibility of Treg based therapy is widely accepted, provided that tailor-made clinical grade procedures for isolation and ex vivo cell handling are available.
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