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Intramedullary nailing is a standard surgical procedure for fixation of proximal femoral fractures, but is associated with considerable radiation exposure for controlling the implant placement, due to the percutaneous insertion technique.
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The procedures for fixation, immunochemistry and imaging were as described previously [16].
The procedures for fixation, immunochemistry and imaging were as described previously (Wu and Luo, 2006).
The basic procedures for fixation and hybridization with coral embryos were carried out as described [ 45].
The less invasive surgical stabilisation (LISS) plate fixation method is an orthopaedic procedure for the fixation of distal femoral fractures.
Mammary gland tumours and tissues were freshly collected via routine dissection procedures and split for fixation, liquid N2 snap-freezing (for molecular analyses), and whole-mount preparation.
To analyze the samples for immunohistochemistry, the same procedures for treatment, fixation, blocking, and probing of tissue with primary antibody were followed.
Cells were embedded in paraffin according to standard laboratory procedures for tissue fixation.
The procedures for methanol fixation and indirect immunofluorescence staining of Tetrahymena cells are described previously (Madireddi et al., 1994).
In summary, we found it feasible to combine the 3 procedures for internal fixation of hip fractures on the STV simulator into a reliable and valid test.
The procedures for cell fixation and antibody reaction to detect the cell markers were performed as previously reported [ 37] using the DPBS with 0.2% Tween® 20 (Sigma) as the permeabilisation solution.
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