Sentence examples similar to procedure for cell fractionation from inspiring English sources

For cell fractionation two methods were used.

For cell fractionation, 107 (IM9) cells were incubated for 10 min with GH and washed with cold PBS.

For cell fractionation, 48 hours after transfection cells were resuspended in fractionation buffer (40 µg/ml digitonin, 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 2.5 mM MgCl2) and incubated for 10 min at room temperature.

For cell fractionation, 8 12 175 cm flasks were collected as above, and washed with PBS.

For cell fractionation studies, sufficient starting material is more readily obtained from 2D cultures.

For cell fractionation, E. coli cells were grown in INV medium at 37°C up to an OD600 of 1.2.

(A ) MRC-5 cells were infected 3 days (TB40-E) or not (NT) with HCMV and harvested for cell fractionation.

The quality of cell fractionation was verified by staining for a mitochondria-specific protein (1∶500, mouse anti-mito-marker, Abcam).

The same procedure was done for cytochrome c following cell fractionation (Abcam, UK) and using anti-cytochrome c antibody (Abcam, UK).

Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation.

For detection of chromatin bound protein, the in situ cell fractionation procedure was performed before fixation [18].