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To analyze the complex procedure effect one should, also, control the non-specific psychological covert intervention by the mean of a pertinent "psychological" control group.
Assessment of c-Fos expression in the hypothalamus (Fig. 3B, right-hand panel) after the completion of spatial recognition did not show any effect of testing procedure either in WT or P2X7R−/− mice (testing procedure effect, F 1,20) = 0.96, n.s.; genotype x testing procedure effect F 1,20) = 0.008, n.s).s
Whatever the ITI, the Y maze experience increased IL-1β expression in the hippocampus (testing procedure effect, ITI 2-min, F 1,20) = 17.7; p<0.001; ITI 30-min, F 1,20) = 5.7; p<0.05.7
As expected, the Y maze exposure did not induce IL-1β mRNA expression in the hypothalamus either in WT or P2X7R−/− mice (testing procedure effect, F 1,20) = 0.98; n.s.; genotype, F 1,20) = 0.83; p<0.01; genotype x testing procedure, F 1,20) = 2.18; n.s).s
The responsiveness was very good for each of the five scales, either after an analgesia and a nociceptive procedure (effect size >0.8).
This protocol was used to acclimatize mice to i.g. administration to prevent potential gavage procedure effect on weight changes (run-in period).
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A restricted least-squares procedure effects the modification so that the actual pattern is as close as possible to the preferred one.
This procedure effected chance on significant findings.
Such interventions can incorporate state of the art theory-based procedures, effect behavior change, and provide opportunities to test theoretical constructs and procedures.
14 The more restrictive procedures effect their changes in part hormonally; laparoscopic sleeve gastrectomy alters ghrelin levels, an effect not seen with gastric banding.
Dialysis patients sit in the clinic a few times a week with other patients experiencing similar procedures, effects, and emotions.
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