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Later, using a discretization procedure, cells will be filled with nodes that represent nameservers.
In the optimized procedure, cells diluted with buffer were stained with 0.24 0.47 μg/ml of Nile red for 5 min, and the fluorescent emission spectra in the wavelength region of 400 to 700 nm excited at 488 nm were acquired before and after the Nile red addition.
After the procedure, "cells proliferated normally.
After the experimental procedure, cells were fixed as described for scanning electron microscopy, dehydrated in increasing concentrations of acetone and flat-embedded in Polybed (Polysciences®).
Intracellular cytokine staining procedure: Cells from blood, spleen or lungs of mice were stimulated for 1 2 h with 2 µg/ml Ag at 37°C and subsequently incubated for 5 h at 37°C with 10 µg/ml brefeldin A (Sigma-Aldrich, Denmark) at 37°C.
During the sorting procedure, cells were kept at 4 °C.
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The experimental procedure, cell assembling and methodology are designed so that there is reasonable consistency with the model assumptions.
By incorporating a low fouling PEG silanization surface modification into the procedure, cell patterning was achieved on the fabricated SWCNT patterns.
By this procedure, cell density in the culture was estimated by measuring optical density (540 nm) to determine whether the Rhizobium culture reached the middle or late logarithmic phase.
Immediately after the isolation procedure, cell morphology and mitochondrial potentials were well preserved, as documented by WGA and TMRM staining).
For hBMEC cells, further optimization was carried out by varying coating material, coating procedure, cell seeding density, and growth media composition.
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