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Three weeks after the IFS procedure, cell proliferation in the dentate gyrus was not longer affected by the IFS procedure: The number of proliferating cells in the EE-continues group was comparable to the number of proliferating cells in the non-shocked EE-continues group and was almost twice as high as in the SH groups (F 1,18) = 36.27, p<0.001).
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After the procedure, "cells proliferated normally.
The effect of the IFS procedure on cell proliferation in the dentate gyrus of the hippocampus was studied in the EE-continues group.
The detailed procedure of cell proliferation (MTT) assay and IC50 determination was described previously.
The toxicity of compounds showing inhibitory effect on T cells was also analyzed using same procedure of T cell proliferation.
However, a limited database and nonstandard protocols and procedures for measuring cell proliferation have made it difficult to compare results between laboratories.
Using this procedure, we determined that cell proliferation was uniform in all IPF and control cell lines, with an approximate doubling time of 1 day.
BrdU uptake procedure showed an increase in cell proliferation in FGF2-treated cultures after 48 h of treatment.
Fig. 3 Schematic views of the cell proliferation test procedure: a dispensing of cell droplets into the well, b addition of the CCK-8 solution into the well and measurement of light absorbance after incubation, and c replacement with fresh cell media.
Briefly, transfection procedure was same as the cell proliferation assays.
Therefore, we emphasize that the impact of sterilization of biomaterials is substantial and any new procedure has to be evaluated by correlating the impact of the procedure on the porous structure with cell proliferation behavior.
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