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Results show that the inclusion of indirect costs (externalities) in the optimization procedure can affect significantly the characteristics of the minimum cost design, the operational conditions and HEN topology.
This is because this procedure can affect the ethanol production itself (or another primary metabolite), apart from directly adding an extra cost to the industrial procedure, where commercial margins are already narrow (Zhao et al. [2009]).
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In this new perspective, flow alterations due to different cardiovascular procedures can affect the long-term outcome of those procedures.
Fixation and permeabilization procedures can affect the outcome of immunocytochemistry experiments.
Both procedures can affect animal well-being and introduce experimental artefacts.
Other parameters, including array and experimental design, protocols and data processing procedures, can affect array data independently of biological processes.
These methods are however important, because variable selection procedures can affect the composition of the multivariable model due to estimation bias, or may result in an increase in model uncertainty [ 72- 74].
We expect that the way in which parents treating their child with T1DM during these often annoying, sometimes even invasive, yet unavoidable procedures can affect blood glucose control (HbA1c) and psychosocial functioning.
Besides using high-quality analytical methods and good laboratory practices for biomonitoring, evaluation of the collection and handling of biological samples should be emphasized, because these procedures can affect the samples integrity and representativeness.
That said, sampling processing procedures can affect this ratio because the phylum Firmicutes consists of mostly gram-positive bacteria with thick cell walls that can make them difficult to lyse, thus high-energy lysis steps (e.g., bead-beating) are important in extraction protocols.
While all these modalities are implemented for in vivo imaging, there are certain differences in terms of animal handling and preparation for imaging, how the monitoring systems are implemented and, importantly, how the imaging procedures themselves can affect mammalian physiology.
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