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The losses are accounted for by the multi-step pre-fractionation procedure, but are not the result of a precipitate that could not be dissolved in our lysis buffer.
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Sham-operated rats underwent the same procedure but were injected with an identical volume (100 μl) of saline only.
In the sham group, mice underwent the same procedure but were neither ligated nor punctured.
Pre-hospital laryngoscopic endotracheal intubation (ETI) is potentially a life-saving procedure but is a technique difficult to acquire.
He said: "That feels utterly at odds with current procedure but was probably not as unusual at that time.
Suctioning of artificial airways is a necessary procedure but is not without risk.
TFFixedIGNot AllowedV2 uses a more biased procedure, but is more computationally tractable.
Control animals underwent the EP procedure but were not injected with the GHRH-plasmid.
One of the 10 patients developed mild pancreatitis after the procedure, but was successfully managed conservatively.
CD69 expression was used as a control for the activation procedure but was not included in the gating strategy.
The leukocyte levels were raised after the first procedure but were reduced before the last session (P < 0.034).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com