Sepsis was induced by the CLP procedure as previously described [22, 23].
The AMT tracer was synthesized using a high-yield procedure as previously outlined [35].
The titanium-oxide coatings were applied in a dip-coating procedure as previously described (Haenle et al. 2011a; Heidenau et al. 2001a, b, 2005).
Postmortem inspection was performed following the procedure as previously described [14].
We used the very same stimuli and procedure as previously described (see [1], condition AB).
Neutralizing antibody titers were determined by microneutralization (MN) assays performed on MDCK cells following the procedure as previously described [22].
FRT cells were stably transfected with a cDNA encoding 3F4 tagged moPrPC with the calcium phosphate procedure, as previously described [53].
In frame deletions of sigT (CC_3475), sigU (CC_2883), phyR (CC_3477), HK4 (CC_3474), CC_3466 and CC_1532 were obtained by a two-step sacB counterselection procedure, as previously described [64].
For that, total proteins were extracted from unstressed and mild stress treated cultures following a similar procedure as previously described [15], [26].
The spontaneous spatial recognition in the Y-maze was used as a hippocampal-dependent test [16] as part of a two-trial procedure, as previously described [17] [20].
Recombinant BAAV, BAAV-HA, AAV4-HA, and AAV4 viruses expressing eGFP were produced using a four-plasmid procedure as previously described [8].
