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The estimation procedure applied was based on a mathematical process model and the measured signals of temperature and refractive index.
In our study, the TTO procedure applied was precisely described, facilitating the comparison with other studies.
The animal treatment procedure applied was under considerations of the Bioethical Committee of the University of Murcia.
The staining procedure applied was a peroxidase method previously described [ 23], with some modifications, as we used endogenous peroxidase block (with 1% H2O2 and 2% NaN3), avidin-biotin (Vector Laboratories, Burlingame, CA, USA) and 1% normal horse serum in blocking steps.
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The procedure applied is based on the work of Kerkhofs and Lindeboom [6] and Jürges [1].
Detailed statistical procedure applied is described in Supplementary Information.
The experimental procedure applied is depicted in Figure 4c.
Analyse-it for Excel (Version 2.11, 2008) was used for the statistical analyses; the statistic procedure applied being the Deming fit.
This study and all the procedures applied were approved by the Institutional Animal Experiment Ethics Committee ("Dierenexperimentencommissie" NKI: DEC-nr. 04089), and all efforts were made to minimize suffering of the animals.
The procedures applied are described in the supplementary information provided online [ 24].
The procedures applied were: Gaps-in-Noise test (GIN), Duration Pattern test, and Phonological Awareness test (PCF).
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