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After completion of imaging procedure, animals were euthanized with pentobarbital overdose.
After the surgical procedure, animals were allowed to stabilize for 30 min (min).
During the procedure, animals were monitored for the presence of spontaneous movement and blood oxygen saturation and if needed, appropriate adjustments to anesthesia were made.
Most animals are used in only one procedure: animals are frequently euthanized after the experiment; however death is the endpoint of some procedures.
During the entire procedure animals were kept on a heating pad.
For the procedure, animals were lightly sedated with 4% isoflurane, and then maintained at 2.0% isoflurane.
Similar(17)
In addition to being a notoriously inefficient procedure, animal cloning has produced many animals with conspicuous developmental problems, like respiratory illnesses and overly large placentas.
Prior to all procedures animals were allowed to acclimatise for at least one week.
For operative procedures, animals were anesthetized with isoflurane inhalation.
For the day 9 and 10 challenge procedures animals were intubated and mechanically ventilated.
Before the experimental procedures, animals were allowed to acclimatize for a week.
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