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The P values were corrected for multiple testing using the Bonferroni procedure and transformed by taking the −log10 for easier visualization [ 40, 41].
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While Chilean officials press for a quick resolution of the situation, Ms Pinochet has several legal options which could keep her in America for months, effectively stalling the deportation procedure and transforming herself into the centrepiece of yet another international Pinochet legal drama.
Electro competent Escherichia coli CLM24 (pGVXN345, pGVXN345) were prepared by standard procedures and transformed with 2 μL of purified mega primer-quick change product using electroporation (1 mm gap width cuvettes, 5 ms pulses of 1.8 kV).
Transgenic plants were generated by Agrobacterium tumefaciens-mediated transformation according to the described procedure [ 35], and transformed lines were first selected for hygromycin (50 mg L-1) resistance and then analyzed by PCR to determine the presence of T-DNA integration.
Competent E. coli LE392 cells were prepared by the calcium chloride procedure [ 28] and transformed with the above described expression plasmids.
For stable transformation, plasmids were digested with SwaI and transformed using standard procedures [13].
Competent cells of B. subtilis GP800.2 were prepared and transformed according to described procedures [ 23].
Although this method performs well for enhancement of LDR images, the image enhancement procedure is transformed to operate in logarithmic domain.
In order to have a reliable and meaningful comparison of gene expression and DNA methylation levels, the values were balanced by a min-max normalization procedure which transformed them to (0,1) range [ 35].
The traditional Tabu Search procedure is then transformed into a hybrid procedure composed of two alternative parts, namely Variable Neighborhood Descent and Tabu Search respectively.
A detailed example as presented in this paper illustrates the whole design procedure and technique to transform the design of a spatial curve into that of two planar curves on two developable surfaces.
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